| Planctonic organisms were collected by sampling from pond water with a sterile glass container, which was immersed and held underwater for at least 5 minutes. The container was closed tightly before being transported to the laboratory. The wet mount prepared from the pond water sample shows two Paramecium cells joined at their oral grooves undergoing conjugation. During conjugation, the exchange of DNA occurs after a series of mitotic and meiotic divisions of micronuclei.
References.
1. Edmondson, W. T. 1959. Freshwater biology, 2nd ed. John Wiley and Sons, Inc., New York, NY.
2. Myohara, K., and K. Hiwatashi. Mutans of sexual maturity in Paramecium caudatum selected by erythromycin resistance. Genetics 90:227–241.
3. Pape, R., B. Haacke-Bell, N. Luthe, and H. Plattner. 1988. Conjugation of Paramecium tetraurelia cells: selective wheat germ agglutinin binding, reversible local trichocyst detachment and secretory function repair. J. Cell Sci. 90:37–49.
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