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Citrate Test Send Print

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Created: Tuesday, 08 December 2009
Last update: Tuesday, 14 December 2010
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Citrate test Citrobacter freundii and Escherichia coli
FIG. 1. A positive citrate test result given by Citrobacter freundii (left) is indicated by growth and a blue color change. A negative citrate test result given by Escherichia coli (right) is indicated by a lack of growth and no color change. (Mary G. Miller, Southeastern Louisiana University, Hammond)   
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Citrate test on Klebsiella pneumoniae and Escherichia coli
FIG. 2. A positive citrate test result given by Klebsiella pneumoniae in the positive control (tube 1) and the test (tube 2) is indicated by growth and a blue color change. A negative citrate test result given by Escherichia coli in the negative control (tube 3) is indicated by a lack of growth and no color change. (Shashidhar Vishwanath, Kasturba Medical College, Karnataka, India)  
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Citrate test on Escherichia coli and Enterobacter aerogenes

FIG. 3. A negative citrate test result given by Escherichia coli (left) is indicated by a lack of growth and no color change. A positive result given by Enterobacter aerogenes (right) is indicated by growth and a blue color change. (Tasha L Sturm, Cabrillo College, Aptos, CA)

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Uninoculated citrate test
FIG. 4. Uninoculated citrate slant. The green color indicates that the medium pH is between 6.9 and 7.6. (Maria P. MacWilliams, University of Wisconsin—Parkside, Kenosha)
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Positive citrate test
FIG. 5. This image depicts a positive citrate test. Note the blue color of the medium and the faint white layer of growth on the slant surface. (Maria P. MacWilliams, University of Wisconsin—Parkside, Kenosha)
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Citrate test on Proteus mirabilis and Klebsiella pneumoniae
FIG. 6. A negative citrate test result given by Proteus mirabilis (left) is indicated by a lack of growth and no color change. A positive result given by Klebsiella pneumoniae (right) is indicated by growth and a blue color change. An uninoculated tube is also shown. The image depicts the results after a 30-hour incubation. (Jackie Peltier Horn, Houston Baptist University, Houston, TX) 
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Citrate test on Proteus mirabilis and Klebsiella pneumoniae
FIG. 7.  A negative citrate test result given by Proteus mirabilis (left) is indicated by a lack of growth and no color change. A positive result given by Klebsiella pneumoniae (right) is indicated by growth and a blue color change. An uninoculated tube is also shown. The image depicts the results after a 30-hour incubation. (Jackie Peltier Horn, Houston Baptist University, Houston, TX)   
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Citrate test on Escherichia coli
FIG. 8. The negative result of a citrate test done on Escherichia coli after a 24-hour incubation at 37°C.  A negative result is indicated by a lack of growth and no color change. (Anne Hanson, University of Maine, Orono)
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Citrate test on Enterobacter aerogenes

FIG. 9. The positive result of a citrate test done on Enterobacter aerogenes after a 24-hour incubation at 37°C.  A positive result is indicated by growth and a blue color change. (Anne Hanson, University of Maine, Orono)

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Citrate test on Escherichia coli and Enterobacter aerogenes
FIG. 10. The image depicts the results of a citrate test after a 24-hour incubation at 37°C. Tube A shows a negative result given by Escherichia coli which is indicated by a lack of growth and no color change. Tube B shows a positive result given by Enterobacter aerogenes which is indicated by growth and a blue color change. (Anne Hanson, University of Maine, Orono)
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