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Endospore Stain Send Print

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Created: Saturday, 29 September 2007
Last update: Tuesday, 23 August 2011
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Author - Secondary
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Spore stain_Endospore stain (Enlarged view)
FIG. 1. Spore stain. (Jerry Keplinger, East Tennessee State University, Johnson City, TN)
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Bacillus sp._Endospore stain (Enlarged view)
FIG. 2. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
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Bacillus sp._Endospore stain (Enlarged view)
FIG. 3. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD) 
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Bacillus sp._Endospore stain (Enlarged view)

FIG. 4. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)

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Bacillus sp._Endospore stain (Enlarged view)

FIG. 5. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)

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Bacillus subtilis_Endospore stain (Enlarged view)
FIG. 6. Endospore-positive Bacillus subtilis. (Ann C. Smith, University of Maryland, College Park, MD)
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Staphylococcus sp._Endospore stain (Enlarged view)
FIG. 7. Endospore-negative Staphylococcus species. (Ann C. Smith, University of Maryland, College Park, MD)  
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Staphylococcus sp._Endospore stain (Enlarged view )
FIG. 8. Endospore-negative Staphylococcus species. (Ann C. Smith, University of Maryland, College Park, MD) 
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Bacillus sp._Endospore stain (Enlarged view)

FIG. 9. Phase-contrast image of endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)

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Bacillus sp._Endospore stain (Enlarged view)

FIG. 10. Phase-contrast image of endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)

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Bacillus sp._Endospore stain (Enlarged view)
FIG. 11. Phase-contrast image of endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD) 
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Bacillus subtilis_Endospore stain (Enlarged view)
FIG. 12. Bacillus subtilis, an endospore-producing bacterium. (Brian Kimble, University of Maryland, College Park, MD)
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Bacillus cereus_Endospore stain (Enlarged view)
FIG. 13. Endospore stain of a Bacillus cereus culture using the Shaeffer-Fulton method and viewed at 1,000x total magnification under an oil immersion lens. Vegetative cells of B. cereus are in red; endospores are in green.  Due to the age of the culture, endospores have been released from the cells.  (Derek Weber and S. Finazzo, Broward Community College–Central campus, Davie, FL)
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Bacillus sp._Endospore stain (Enlarged view)

FIG. 14. Bacillus spp. stained with Sheaffer-Fulton technique showing green endospores. (Javier Gutierrez Jimenez, Sciences and Arts University of Chiapas, Mexico)

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Bacillus sp._Endospore stain (Labeled view)

FIG. 14. Bacillus spp. stained with Sheaffer-Fulton technique showing green endospores. (Javier Gutierrez Jimenez, Sciences and Arts University of Chiapas, Mexico)

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Bacillus subtilis_Endospore stain(Enlarged view)
FIG. 15. Bacillus subtilis. (Alan Schenkel, Peter Justice and Erica Suchman, Colorado State University, Fort Collins, CO)
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Bacillus subtilis_Endospore stain(Enlarged view)
FIG. 16. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
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Bacillus subtilis _Endospore stain(Enlarged view)
FIG. 17. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
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Bacillus subtilis _Endospore stain(Enlarged view)
FIG. 18. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
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Bacillus subtilis _Endospore stain(Enlarged view)
FIG. 19. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO) 

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Information

Unless otherwise stated all images in this collection show cells that were visualized at 1000X magnification using Brightfield microscopy. Various camera settings have magnified the images further.

Endospore Stain Spanish Translation

 
REVIEWERS

This resource was peer reviewed at the ASM Conference for Undergraduate Educators 2007. 
 
Participating reviewers:
 
Cindy Anderson
Mt. San Antonia College, Walnut, CA

Jennifer Brofft
Armstrong Atlantic State University, Savannah, GA

Vicki Huffman
Potomac State College, Keyser, WV
 
Lucy Kluckhohn Jones
Santa Monica College, Santa Monica, CA
 
Shawn Lester
Montgomery College, Rockville, MD
 
Robin Maxwell
The University of North Carolina at Greensboro, Greensboro, NC
 
Carl F. McAllister
Georgia Perimeter College, Clarkston, GA
 
Ruth Ann Mikels
Tacoma Community College, Tacoma, WA
 
Linda Navitsky
Tunxis Community College, Farmington, CT
 
Frances Sailer
University of North Dakota, Grand Forks, ND
 
Kathryn Sutton
Clarke College, Dubuque, IA
 
Christine Weingart
Denison University, Granville, OH
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