| Blood Agar Plates and Hemolysis: Streptococcus and Other Catalase Negative Gram-Positive Cocci |  |
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| Created: Friday, 30 September 2005 |
| Last update: Wednesday, 06 July 2011 |
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 Typticase soy agar w/ & w/o sheep blood (Enlarged View) | FIG. 1. Tryptic soy agar with and without Sheep Blood. (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus pyogenes (Enlarged View) | FIG. 2. Beta hemolytic Streptococcus species seen with transmitted light, Streptococcus pyogenes (Lancefield group A. Also see Figures 28-35). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Normal respiratory flora with Streptococcus (Enlarged View) | FIG. 3. Normal Upper respiratory flora mixed with Streptococcus species. (The presence of beta-hemolytic colonies indicates the possibility of Streptococcus pyogenes infection. Also see Figures 26 and 27). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus pyogenes (Enlarged View) | FIG. 4. Same blood agar plate as Figure 2 demonstrating that the beta hemolysis of Streptococcus pyogenes is so complete that print my be read through the resulting transparent medium. (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Normal flora with Streptococcus pyogenes (Enlarged View) | FIG. 5. Normal Upper respiratory flora mixed with Streptococcus pyogenes demonstrating production of Streptolysin O. Beta hemolysis is only evident where the agar was "stabbed". (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Normal flora with Streptococcus pyogenes (Enlarged View) | FIG. 6. Normal Upper respiratory flora mixed with Streptococcus pyogenes demonstrating production of Streptolysin O. Beta hemolysis is only evident where the agar was "stabbed". (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus agalactiae (Enlarged View) | FIG. 7. Streptococcus agalactiae (Lancefield group B) viewed with incident light: No obvious hemolysis. (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus agalactiae (Enlarged View) | FIG. 8. Streptococcus agalactiae (Lancefield group B) viewed with transmitted light: Subtle hemolysis. (Also see Figure 36 and 37). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Listeria monocytogenes (Enlarged View) | FIG. 9. Listeria monocytogenes, removing colonies to see the subtle hemolysis directly beneath the colonies. (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus species "Viridans group" (Enlarged View) | FIG. 10. Alpha-hemolytic Streptococcus species "Viridans group" streptococci, including species such as the Streptococcus mutans, mitis, and salivarius groups display alpha hemolysis. (Also see Figures 15,16 and 23-25). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus pneumoniae (Enlarged View) | FIG. 11. Alpha hemolysis of Streptococcus pneumoniae (Encapsulated strain, also see Figure 17-20). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Enterococcus faecalis (Enlarged View) | FIG.12. "Gamma Streptococcus": Enterococcus faecalis (24 hours, non-hemolytic). "Gamma streptococci" are usually non-hemolytic after 24 hours of incubation, but many eventually display weak alpha hemolysis. (The genus Enterococcus was once a part of the Streptococcus genus, was considered a "gamma Streptococcus species," and usually reacts as Lancefield group D). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Enterococcus faecalis (Enlarged View) | FIG. 13. The same Enterococcus strain as Figure (12), shown with transmitted light at 48 hours incubation demonstrates the alpha hemolysis of some "gamma streptococci." (See also Figure 38). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Alpha, Beta and Gamma Streptoccoci (Enlarged View) | FIG. 14. Alpha (a), Beta (b) and Non-hemolytic (Gamma, g) streptococci (transmitted light). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus species Viridans group (Enlarged View) | FIG. 15. Alpha-hemolytic Streptococcus species ("Viridans group"). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus species Viridans group (Enlarged View) | FIG. 16. Alpha-hemolytic Streptococcus species ("Viridans group"). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus pneumoniae (Enlarged View) | FIG. 17. In addition to alpha hemolysis, this strain of Streptococcus pneumoniae is producing abundant polysaccharide capsular material evidenced by the mucoid or "oil droplet" appearance on the colonies. (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus pneumoniae (Enlarged View) | FIG. 18. Streptococcus pneumoniae (encapsulated strain). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus pneumoniae (Enlarged View) | FIG. 19. Streptococcus pneumoniae (encapsulated strain). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
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 Streptococcus pneumoniae (Enlarged View) | FIG. 20. Streptococcus pneumoniae (encapsulated strain). (Rebecca Buxton, University of Utah, Salt Lake City, UT) |
Showing results 1-20 of 43 total results
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Blood Agar Plates and Hemolysis Spanish Translation
REVIEWERS This resource was peer-reviewed at ASM Conference for Undergraduate Education 2005. Participating reviewers:
Samuel Fan Bradley University, Peoria, IL Ashalla Freeman University of North Carolina, Chapel Hill, NC Roxana Hughes UNT Biological Sciences, Denton, TX D. Sue Katz Rogers State University, Claremore, OK Lucy Kluckhohn Jones Santa Monica College, Santa Monica, CA Patricia Shields University of Maryland, College Park, MD Erica Suchman Colorado State University, Ft. Collins, CO |
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