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IMViC tests of E. coli Send Print

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Created: Friday, 01 January 2010
Last update: Tuesday, 03 August 2010
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IMViC tests of E. coli
FIG. 4. Results of an IMViC series done on Escherichia coli after a 24-hour incubation at 37°C. Tube A shows a positive indole test in tryptone broth. The positive result is indicated by the red layer at the top of the tube after the addition of Kovács reagent. Tube B shows a positive methyl red test as indicated by the red color after the addition of methyl red reagent. Tube C shows a negative Voges-Proskauer test as indicated by the lack of color change after the addition of Barritt’s A and Barritt’s B reagents. Tube D shows a negative citrate result as indicated by the lack of growth and color change in the tube. (Anne Hanson, University of Maine, Orono, ME)