Taqman quantitative reverse transcription PCR analyzing mRNA levels of IFN-γ, IL-12, and TNF-α in mouse brains following challenge with aerosolized La Crosse virus.  Mice were either left untreated or received cationic liposome-DNA complex (CLDC) immunotherapy prior to virus challenge.  IFN-γ mRNA production was significantly increased in the brains of CLDC-treated mice at both days 2 and 4 postchallenge.  There were no significant differences between the production of IL-12 and TNF-α mRNA in the brains of untreated or treated mice. The orange line is the chosen fluorescence threshold (cycle threshold or Ct value).  (Erik Arthun, Colorado State University, Ft. Collins, CO)