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Escherichia coli in a TSI Slant Send Print

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Created: Thursday, 01 November 2012
Last update: Thursday, 01 November 2012
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Escherichia coli in a TSI Slant

Escherichia coli in a triple sugar iron (TSI) slant. This medium contains glucose, lactose, and sucrose.  Escherichia coli ferments glucose and lactose producing acid and carbon dioxide.  Acid causes the phenol red indicator in the agar to turn yellow.  Carbon dioxide is observed as bubbles or cracks in the agar. There is no hydrogen sulfide production, as indicated by the lack of black precipitate in the agar. (Diane Hartman, Baylor University, Waco, TX)