Microscopic Examinations of Skin Infections—Filamentous Fungi (Dermatophytes) - Library

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Microscopic Examinations of Skin Infections—Filamentous Fungi (Dermatophytes)

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Created: Tuesday, 06 February 2007

Last update: Wednesday, 28 September 2011

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Rebecca Buxton (Corresponding Author)

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Dermatophytes (Enlarged view)	Slide 1. Dermatophyte In this potassium hydroxide (KOH) preparation of skin scrapings, thick, septate, branching hyphae are visible. The fainter, thinner, circular lines in the background are the walls of the skin cells (squames). The fungal hyphae are much thicker and more retractile than the cell walls of the squames, over which they cross and with which they are often confused by inexperienced examiners. The three genera of dermatophytes, Microsporum, Trichophyton, and Epidermophyton, are indistinguishable in KOH preparations.
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Trichophyton tonsurans (Enlarged view)	Slide 2. Trichophyton tonsurans This KOH preparation shows arthrospores in a hair shaft.
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Trichophyton rubrum (Enlarged view)	Slide 3. Trichophyton rubrum This KOH preparation of a fingernail scraping shows several retractile septate hyphae, some of which have broken into arthrospores.
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Dermatophytes (Enlarged view)	Slide 4. Dermatophyte Fluorescent microscopy of skin scrapings treated with calcofluor white for chitin in the fungal cell wall readily reveals dermatophytes. The slide clearly demonstrates the septae within the hyphae.

Information	These images are from the original published atlas: Tenover, F. C., and J. V. Hirschmann. 1990. Interpretation of Gram stains and other common microbiologic slide preparations. The UpJohn Company, Kalamazoo, Mich. Permission granted to the ASM MicrobeLibrary by Pfizer Inc. This atlas was written to help clinicians, microbiologists, and laboratory personnel identify organisms in infected materials stained by techniques commonly used in most clinical laboratories. Please refer to the atlas' main page for more information and a guide to all of the images.
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