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Examination of Gram Stains of Urine Send Print

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Created: Tuesday, 06 February 2007
Last update: Wednesday, 28 September 2011
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Escherichia coli (Enlarged view)

Slide 1. Escherichia coli
This Gram stain shows neutrophils and numerous plump, gram-negative bacilli that vary little in size or shape.

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Proteus mirabilis (Enlarged view)

Slide 2. Proteus mirabilis
These short to medium-long gram-negative bacilli look like typical enteric gram-negative bacteria; isolation of Proteus mirabilis confirmed that impression.

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Citrobacter diversus (Enlarged view)

Slide 3. Citrobacter diversus
These short, slender gram-negative bacilli look like enteric bacteria. The culture grew Citrobacter diversus.

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Acinetobacter baumanii (Enlarged view)

Slide 4. Acinetobacter baumanii
Some of the short, fat, pleomorphic gram-negative bacilli resemble cocci, others diplococci. The variety of shapes is typical of this organism, which can be confused with Neisseria species.

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Lactobacillus species (Enlarged view)

Slide 5. Lactobacillus species
These long, slender gram-positive bacilli are part of the normal vaginal flora. Their presence in a urine specimen suggests that improper technique in its collection resulted in contamination with vaginal secretions.

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Staphylococcus saprophyticus (Enlarged view)

Slide 6. Staphylococcus saprophyticus
These gram-positive cocci in clusters look like staphylococci. Various species of coagulase-negative staphylococci, especially Staphylococcus saprophyticus, can cause urinary tract infections, particularly in young women.

Information

These images are from the original published atlas: Tenover, F. C., and J. V. Hirschmann. 1990. Interpretation of Gram stains and other common microbiologic slide preparations. The UpJohn Company, Kalamazoo, Mich. Permission granted to the ASM MicrobeLibrary by Pfizer Inc. 

This atlas was written to help clinicians, microbiologists, and laboratory personnel identify organisms in infected materials stained by techniques commonly used in most clinical laboratories. Please refer to the atlas' main page for more information and a guide to all of the images.

Gram stains of urine specimens that have not been centrifuged can help determine the cause of a suspected urinary tract infection. If bacteria are present under an oil immersion lens (1,000x), the concentration is at least 104 or 105 bacteria per milliliter. Since the concentration of bacteria in most patients with symptomatic urinary tract infections is that high, the Gram stain will usually, but not always, be positive.
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Tags: Microorganisms and humans Microbial cell biology (1) , Microorganisms and humans (1) , Microbial cell biology (1)


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