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Cytopathic Effects of Viruses: Group 1 Infected Bovine Fetal Spleen Cells, Unstained Send Print

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Created: Saturday, 29 September 2007
Last update: Monday, 22 August 2011
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Bovine enterovirus CPE (Enlarged view)
FIG. 1. Unstained bovine fetal spleen cells 1 day postinfection with a high multiplicity of infection (MOI) of bovine enterovirus, a Picornavirus, showing pyknotic shrinking cells. Cells were observed using the 10X objective. Note: this shows viral CPE within 24 hours, within 48 hours all of the cells will be dead and detached. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO) 
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Vesicular stomatitis virus CPE (Enlarged view)

FIG. 2. Unstained bovine fetal spleen cells 2 days postinfection with a high MOI of vesicular stomatitis virus, a Rhabdovirus, showing subtotal cell destruction; the cells appear pyknotic. Cells were observed using the 10X objective. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

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Bovine herpesvirus-1 CPE (Enlarged view)
FIG. 3. Unstained bovine fetal spleen cells 2 days postinfection with a high MOI of bovine herpesvirus 1, a Herpesvirus. Cells were observed using the 10X objective. Black arrows point to cell rounding in a focal pattern and blue arrows point to cytoplasmic stranding. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO) 
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Orf virus CPE (Enlarged view)
FIG. 4. Unstained bovine fetal spleen cells 2 days postinfection with a high MOI of Orf virus, a Parapoxvirus. Blue arrows point to cell rounding in a focal pattern. Cells were observed using the 10X objective. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)  
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Parainfluenza virus-3 CPE (Enlarged view)
FIG. 5.Unstained bovine fetal spleen cells infected with the parainfluenza virus type 3, a Paramyxovirus, showing a syncytium (arrows). Cells were observed using the 10X objective. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Black and white unstained cells  
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Orf virus CPE (Enlarged view)

FIG. 6. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a low MOI. Cells were observed using a 4X objective. Note: you can see the CPE is focal at the low MOI, but it appears generalized at the high MOI. (Erica Suchman, Colorado State University, Fort Collins, CO)

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Orf virus CPE (Enlarged view)
FIG. 7. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a high MOI. Cells were observed using a 4X objective. Note: you can see the CPE is focal at the low MOI, but it appears generalized at the high MOI. (Erica Suchman, Colorado State University, Fort Collins, CO)
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Orf virus CPE (Enlarged view)
FIG. 8. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a low MOI. Cells were observed using a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)
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Orf virus CPE (Enlarged view)

FIG. 9. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a high MOI. Cells were observed using a 10X objective. Note: the focal nature of the infection is easier to see under lower power magnification. (Erica Suchman, Colorado State University, Fort Collins, CO)

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Bovine adenovirus CPE (Enlarged view)

FIG. 10. Unstained bovine fetal spleen cells 4 days postinfection with a high MOI of bovine adenovirus, an Adenovirus, showing cell rounding and small amounts of clumping. Cells were observed using the 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)

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Bovine viral diarrhea virus CPE (Enlarged view)
FIG. 11. Unstained bovine fetal spleen cells 3 days postinfection with a high MOI of bovine viral diarrhea virus, a Flavivirus, showing generalized cell destruction and vacuoles, which are very difficult to see without staining. Cells were observed with a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)
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Bovine viral diarrhea virus CPE (Enlarged view)

FIG. 12. Unstained bovine fetal spleen cells 3 days postinfection with a lower MOI than found in Fig. 11 of bovine viral diarrhea virus, a Flavivirus, showing generalized cell destruction and vacuoles, which are very difficult to see without staining. Cells were observed with a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)

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Bovine parvovirus-4 CPE (Enlarged view)
FIG. 13. Unstained bovine fetal spleen cells 2 days postinfection with a high MOI of bovine parvovirus-4, a Parvovirus, showing generalized cell shrinkage (pyknosis) and cell destruction. Cells were observed with a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)
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Bovine parvovirus-4 CPE (Enlarged view)
FIG. 14. Unstained bovine fetal spleen cells 2 days postinfection with a low MOI of bovine parvovirus-4, a Parvovirus, showing generalized cell shrinkage (pyknosis) and cell destruction. Cells were observed with a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)
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Ovine parvovirus CPE (Enlarged view)
FIG. 15. Unstained bovine fetal spleen cells 4 days postinfection with a high MOI of ovine parvovirus, a Parvovirus, showing generalized cell rounding and cell destruction. Cells were observed with a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)
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Ovine parvovirus CPE (Enlarged view)

FIG. 16. Unstained bovine fetal spleen cells 4 days postinfection with a low MOI of ovine parvovirus, a Parvovirus, showing generalized cell rounding (black arrows) and cell destruction (green arrows). Cells were observed with a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)

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Bovine adenovirus CPE (Enlarged view)

FIG. 17. Unstained bovine fetal spleen cells 4 days postinfection with a high MOI of bovine adenovirus, an Adenovirus, showing generalized enlarged cell rounding and cell clumping or clustering. Cells were observed with a 10X objective. Note, if observed very early in the infection, CPE will begin focally but rapidly becomes generalized. (Erica Suchman, Colorado State University, Fort Collins, CO)

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Bovine adenovirus CPE (Enlarged view)

FIG. 18. Unstained bovine fetal spleen cells 4 days postinfection with a low MOI of bovine adenovirus, an Adenovirus, showing generalized enlarged cell rounding and cell clumping or clustering. Cells were observed with a 10X objective. Note, if observed very early in the infection, CPE will begin focally but rapidly becomes generalized. (Erica Suchman, Colorado State University, Fort Collins, CO)

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Bovine herpes virus-2 CPE (Enlarged view)
FIG. 19. Unstained bovine fetal spleen cells 1 day postinfection with a high MOI of bovine herpes virus-2, a Herpesvirus, showing rapidly occurring focal cell fusion forming syncytium or polykaryons. Cells were observed with a 10X objective. Note: at a high MOI the entire monolayer becomes one large syncytium and begins peeling off the plate, as is observed here. (Erica Suchman, Colorado State University, Fort Collins, CO)
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Bovine herpes virus-2 CPE (Enlarged view)
FIG. 20. Unstained bovine fetal spleen cells 1 day postinfection with a low MOI of bovine herpes virus-2, a Herpesvirus, showing rapidly occurring focal cell fusion forming syncytia or polykaryons. Cells were observed with a 10X objective. Note: at a low MOI the focal nature of the syncytia is more readily observed than at a higher MOI. (Erica Suchman, Colorado State University, Fort Collins, CO)

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REVIEWERS

This resource was peer-reviewed at the ASM Conference for Undergraduate Educators 2007.

Participating reviewers:
 
Suzanne Bassett
Spokane Community College, Spokane, WA

Sandra Burnett

Brigham Young University, Provo, UT

Lana Lamb

University of Minnesota—Twin Cities, Minneapolis, MN

Patricia Shields

University of Maryland, College Park, MD

Carolyn Stenbak

Trinity University, San Antonio, TX

Harshad Thacore
State University of New York at Buffalo, Buffalo, NY

John Trimble
St. Francis University, Loretto, PA

Cytopathic Effects of Viruses: Group 1 Infected Bovine Fetal Spleen Cells, Unstained Spanish Translation
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