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Reverse CAMP Test for the Identification of Clostridium perfringens Send Print

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Created: Monday, 09 October 2006
Last update: Tuesday, 10 August 2010
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Clostridium perfringens Streptococcus agalactiae (Enlarged view)
FIG. 1. Close-up view of a positive reverse CAMP reaction. The test organism, Clostridium perfringens, is streaked down the center of the plate. Streptococcus agalactiae (group B) is streaked at right angles to the Clostridium perfringens. A positive reverse CAMP result for Clostridium perfringens is shown by the arrow-shaped zone of enhanced hemolysis pointing towards Clostridium perfringens. (Anne Hanson, University of Maine, Orono, ME)
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Clostridium perfringens Streptococcus agalactiae (Enlarged view)
FIG. 2. Reverse CAMP-positive Clostridium perfringens is streaked at right angles to Streptococcus agalactiae (group B), which is streaked down the center of the plate. The positive reaction of Clostridium perfringens is shown by the “bow-tie” zone of enhanced hemolysis pointing towards Streptococcus agalactiae (group B). (Anne Hanson, University of Maine, Orono, ME)
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Clostridium perfringens, Clostridium septicum, Streptococcus agalactiae (Enlarged view)
FIG. 3. Reverse CAMP test showing both positive and negative results. Streptococcus agalactiae (group B) is streaked down the center of the plate the test organisms, Clostridium sp., are streaked at right angles to the Streptococcus agalactiae (group B). The positive reverse CAMP reaction of Clostridium perfringens is shown by the “bow-tie" zone of hemolysis. The negative reverse CAMP reaction of Clostridium septicum shows no hemolysis. (Anne Hanson, University of Maine, Orono, ME)
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Clostridium perfringens Streptococcus agalactiae (Enlarged view)
FIG. 4. Close-up view of the positive reverse CAMP reaction demonstrated by Clostridium perfringens when tested against group B Streptococcus. Note the bow-tie zone of enhanced hemolysis pointing towards the Streptococcus agalactiae (group B). (Anne Hanson, University of Maine, Orono, ME)
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REVIEWERS

This resource was peer-reviewed at ASM Conference for Undergraduate Education 2006.

Participating reviewers:

D’Maris Allen-Mierl
Austin Community College, Austin, TX

Rebecca Buxton
University of Utah, Salt Lake City, UT

Lucy Kluckhohn Jones
Santa Monica College, Santa Monica, CA

Dawn Madl
NE Wisconsin Technical College, Green Bay, WI

Anne Mason
Mesa Community College, Mesa, AZ

Johana Melendez
Hillsborough County Community College, Plant City, FL

Peter Milligan
University of Maine at Augusta, Augusta, ME

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