Search

Resource Type
 Video (49)
 Image (439)
 Animation (126)
 Protocol (38)
Tags
 Cell Biology (248)
 Genetics (78)
Humans (363)
 Environment (270)
 Diversity (106)
 Teaching (344)
Banner

Sputum–Gram-Negative Bacilli Send Print

  Votes (0) | Hits (5495) | Comments (0)
Created: Tuesday, 06 February 2007
Last update: Tuesday, 27 September 2011
Author
View / Comment
Preview
Escherichia coli (Enlarged view) Enlarged view
Slide 1. Escherichia coli
These gram-negative bacilli vary little in size and shape in this stained preparation and are “plumper” than Haemophilus influenzae.
Because the ends of some bacilli stain more densely than does the middle (a characteristic called “bipolar staining”), the bacilli
resemble safety pins. Bipolar staining is common in enteric gram-negative bacilli, and if not carefully examined, the organisms may appear to be diplococci rather than bacilli. Several bacteria are present in the cytoplasm of neutrophils.
View / Comment
Preview
Enterobacter cloacae (Enlarged view)

Slide 2. Enterobacter cloacae
The plump, gram-negative bacilli vary somewhat in size. Enteric gram-negative bacilli, such as Enterobacter, Klebsiella, and Escherichia coli, are difficult to differentiate by their appearance on Gram stain.

View / Comment
Preview
Pseudomonas aeruginosa (Enlarged view)

Slide 3. Pseudomonas aeruginosa
These long, slender, gram-negative bacilli are nearly uniform in size. Pseudomonas species tend to be longer and slimmer than enteric gram-negative bacilli, such as Klebsiella and Escherichia coli, and bipolar staining is unusual. Despite such differences, however, distinguishing Pseudomonas species from other gram-negative bacilli is often difficult.

View / Comment
Preview
Stenotrophomonas maltophilia (Enlarged view)

Slide 4. Stenotrophomonas maltophilia
This specimen was obtained by bronchoscopy from a neutropenic recipient of a bone-marrow transplant. Neutrophils are absent, but the presence of ciliated bronchial epithelial cells confirms the lower respiratory tract as the source. Ciliated bronchial epithelial cells are rarely found in expectorated sputum but are common in bronchoscopic specimens. Thin, long, faintly-stained bacilli could suggest a species of Pseudomonas or Stenotrophomonas maltophilia.

View / Comment
Preview
Acinetobacter baumanii (Enlarged view)

Slide 5. Acinetobacter baumanii
Many of the plump, very short, gram-negative bacilli are nearly coccal. Their appearance is characteristic of Acinetobacter species, although they are sometimes mistakenly identified as Neisseria species.

Information

These images are from the original published atlas: Tenover, F. C., and J. V. Hirschmann. 1990. Interpretation of Gram stains and other common microbiologic slide preparations. The UpJohn Company, Kalamazoo, Mich. Permission granted to the ASM MicrobeLibrary by Pfizer Inc.

 
This atlas was written to help clinicians, microbiologists, and laboratory personnel identify organisms in infected materials stained by techniques commonly used in most clinical laboratories. Please refer to the atlas' main page for more information and a guide to all of the images.
Related Content
ShareIcon Share





Tags: Cell biology (250) , Microbes in humans (373)


There are no comments for this item

Be the first to leave a comment

Login to leave a comments