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Cytopathic Effects of Viruses: Group 3 Giemsa-Stained Infected Cell Lines Other than Bovine Fetal Spleen Cells Send Print

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Created: Saturday, 29 September 2007
Last update: Tuesday, 23 August 2011
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Feline herpesvirus-1 CPE (Enlarged view)
FIG. 1. Giemsa-stained feline nasal turbinate primary cell culture infected with feline herpesvirus-1, a Herpesvirus, showing chromatin margination (dark ring around the edge of the nucleus). Cells were observed with a 20X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Feline herpesvirus-1 CPE (Enlarged view)
FIG. 2. Giemsa-stained feline nasal turbinate primary cell culture infected with feline herpesvirus-1, a Herpesvirus, showing chromatin margination (dark ring around the edge of the nucleus). Cells were observed with a 40X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Bovine enterovirus CPE (Enlarged view)
FIG. 3. May-Grunwald Giemsa-stained bovine fetal lung cells 48 hours postinfection with bovine enterovirus (Picornaviridae) showing cell destruction, but no inclusions. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)

 

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Bovine herpesvirus-1 CPE (Enlarged view)
FIG. 4. Giemsa-stained bovine nasal turbinate cells 16 hours postinfection with bovine herpesvirus-1 (IBR) showing nuclear inclusions and cytoplasmic stranding. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Sindbis virus CPE (Enlarged view)
FIG. 5. Giemsa-stained Vero cells (from an African green monkey kidney) 24 hours postinfection with Sindbis virus (Alphavirus) showing cell destruction and pyknosis. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Sindbis virus CPE (Enlarged view)
FIG. 6. Giemsa-stained Vero cells (from an African green monkey kidney) 48 hours postinfection with Sindbis virus (Alphavirus) showing cell destruction and pyknosis. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Herpes simplex virus-1 CPE (Enlarged view)
FIG. 7. Giemsa-stained Vero cells (from an African green monkey kidney) 30 hours postinfection with herpes simplex virus-1 showing cell rounding and clustering. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Human reovirus CPE (Enlarged view)
FIG. 8. Giemsa-stained Vero cells (from an African green monkey kidney) 9 days postinfection with human reovirus showing basophilic, perinuclear, cytoplasmic inclusions. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Feline herpesvirus-1 CPE (Enlarged view)
FIG. 9. Giemsa-stained Crandall feline kidney cells 24 hours postinfection with feline herpesvirus-1 showing cell rounding and cytoplasmic stranding. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Feline herpesvirus-1 CPE (Enlarged view)
FIG. 10. Giemsa-stained Crandall feline kidney cells 48 hours postinfection with feline herpesvirus-1 showing cell rounding, nuclear inclusions, and cytoplasmic stranding. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Human adenovirus CPE (Enlarged view)
FIG. 11. Giemsa-stained HeLa cells (from a human cervical carcinoma) 5 days postinfection with human adenovirus showing cell rounding and clustering. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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Human adenovirus CPE (Enlarged view)
FIG. 12. Giemsa-stained HeLa cells (from a human cervical carcinoma) 7 days postinfection with human adenovirus showing pronounced cell enlargement, rounding, and distinctive “grape-like" clusters. Cells were observed with a 10X objective. (Erica Suchman and Carol Blair, Colorado State University, Fort Collins, CO)
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REVIEWERS

This resource was peer-reviewed at the ASM Conference for Undergraduate Educators 2007.

Participating reviewers:
Suzanne Bassett
Spokane Community College, Spokane, WA
 
Sandra Burnett
Brigham Young University, Provo, UT
 
Lana Lamb
University of Minnesota—Twin Cities, Minneapolis, MN
 
Patricia Shields
University of Maryland, College Park, MD
 
Carolyn Stenbak
Trinity University, San Antonio, TX
 
Harshad Thacore
State University of New York at Buffalo, Buffalo, NY
 
John Trimble
St. Francis University, Loretto, PA
  

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