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Cytopathic Effects of Viruses: Group 4 Unstained Infected Cell Lines Other than Bovine Fetal Spleen Cells Send Print

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Created: Saturday, 29 September 2007
Last update: Tuesday, 23 August 2011
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Equine herpesvirus-1 CPE (Enlarged view)

FIG. 1. Unstained neuroblastoma (SK-N-SH) cell monolayer 72 hours postinfection at low multiplicity of infection (MOI) with equine herpesvirus type 1 showing cell rounding and retraction from the substrate leaving gaps in the monolayer. Cells were observed using phase-contrast microscopy at 128X magnification. (Emmanuel Brako, Winona State University, Winona, MN and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)

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Equine herpesvirus-1 CPE (Enlarged view)

FIG. 2. Unstained rat lung fibroblast monolayer 72 hours postinfection at low MOI with equine herpesvirus type 1 showing cell destruction as characterized by cell rounding and retraction from the substrate leaving gaps in the monolayer. Cells were observed using phase-contrast microscopy at 128X magnification. (Emmanuel Brako, Winona State University, Winona, MN  and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)

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Equine herpesvirus-1 CPE (Enlarged view)

FIG. 3. Unstained rabbit kidney (LLC-RK1) cell monolayer 6 hours postinfection at low MOI with equine herpesvirus type 1 showing a focal area of polykaryocyte (also called syncytium or giant cell) (green arrow).  Cells were observed using phase-contrast microscopy at 320X magnification. (Emmanuel Brako, Winona State University, Winona, MN  and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)

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Equine herpesvirus-1 CPE (Enlarged view)

FIG. 4. Unstained rabbit kidney (LLC-RK1) cell monolayer 24 hours postinfection at low MOI with equine herpesvirus type 1 showing a focal area of polykaryocyte (also called syncytium or giant cell).  Cells were observed using phase-contrast microscopy at 320X magnification. (Emmanuel Brako, Winona State University, Winona, MN  and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)

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REVIEWERS

This resource was peer-reviewed at the ASM Conference for Undergraduate Educators 2007.

Participating reviewers:

Suzanne Bassett

Spokane Community College, Spokane, WA

Sandra Burnett

Brigham Young University, Provo, UT

Lana Lamb

University of Minnesota—Twin Cities, Minneapolis, MN

Patricia Shields

University of Maryland, College Park, MD

Carolyn Stenbak

Trinity University, San Antonio, TX

Harshad Thacore
State University of New York at Buffalo, Buffalo, NY

John Trimble
St. Francis University, Loretto, PA
 

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