FIG. 1. Three sector T streak. Serratia marcescens, strain Nima, on trypticase soy agar. Water on agar surface allowed culture to spread across the surface of the agar. (D. Sue Katz, Rogers State University, Claremore, OK)
| Streak Plate: Illustrations of Common Problems with Streak Plates |  |
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| Created: Monday, 08 September 2008 |
| Last update: Tuesday, 10 August 2010 |
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 Serratia marcescens common problems (Enlarged view) | |
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 Serratia marcescens common problems (Enlarged view) | FIG. 2. Three sector T streak. Serratia marcescens, strain Nima, on trypticase soy agar. Water on agar surface allowed culture to spread across the surface of the agar. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Serratia marcescens common problems (Enlarged view) | FIG. 3. Three sector T streak. Serratia marcescens, strain Nima, on trypticase soy agar. Water Petri dish rim, allowed the culture to spread around the edge of the plate. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Serratia marcescens common problems (Enlarged view) | FIG. 4. Three sector T streak. Serratia marcescens on trypticase soy agar. Isolation was achieved, although the first sector's streaking is very heavy and overlapping. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Escherichia coli common problems (Enlarged view) | FIG. 5. Three sector T streak. Escherichia coli on trypticase soy agar. The first and second sectors were not streaked closely together. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Serratia marcescens common problems (Enlarged view) | FIG. 6. Three sector T streak. Serratia marcescens, strain Nima, on trypticase soy agar. The loop was not flamed adequately to prevent carryover of bacteria from one sector to the next. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Serratia marcescens common problems (Enlarged view) | FIG. 7. Three sector T streak. Serratia marcescens, strain Nima, on trypticase soy agar. Water was present on the rim of the plate, and while isolation was achieved, bacteria are spread around the rim of the plate. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Escherichia coli common problems (Enlarged view) | FIG. 8. Three sector T streak. Escherichia coli on trypticase soy agar. The streak pattern failed to use sufficient area of the plate. Additionally, it appears that improper sterilization of the loop allowed between sectors. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Escherichia coli common problems (Enlarged view) | FIG. 9. Four quadrant streak. Escherichia coli on trypticase soy agar. Bacteria were brought from the first sector into the fourth sector when the loop touched into the first quadrant. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Serratia marcescens common problems (Enlarged view) | FIG. 10. Three sector T streak. Serratia marcescens on trypticase soy agar. Water on agar surface allowed culture to spread across the surface of the agar (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Serratia marcescens common problems (Enlarged view) | FIG. 11. Three sector T streak. Serratia marcescens on trypticase soy agar. The streak pattern for sectors 2 and 3 indicates that the loop was not adequately flamed. Additionally, bacteria were brought into the third streak sector from the first sector. (D. Sue Katz, Rogers State University, Claremore, OK) |
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 Escherichia coli common problems (Enlarged view) | FIG. 12. Three sector T streak. Escherichia coli plated onto trypticase soy agar. Although isolated colonies were obtained, the streaking on the first sector is sparse and the loop was not adequately sterilized to diminish the amount of bacteria carried to the second sector. (D. Sue Katz, Rogers State University, Claremore, OK) |
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| Tags: Cell biology (251) , Microbial genetics (80) , Microbes in humans (376) , Microbial evolution and diversity (108) , Teaching and learning (361) |