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Phenylethyl Alcohol Agar Send Print

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Created: Tuesday, 01 November 2011
Last update: Monday, 31 October 2011
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Phenylethyl Alcohol Agar Inoculated with Salmonella enteritidis

Phenylethyl alcohol (PEA) agar plate inoculated with Salmonella enteritidis, a gram-negative coliform bacterium, showing no growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

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Phenylethyl Alcohol Agar Inoculated with Enterobacter aerogenes

Phenylethyl alcohol (PEA) agar plate inoculated with Enterobacter aerogenes, a gram-negative coliform bacterium, showing inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

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Phenylethyl Alcohol Agar Inoculated with Psuedomonas aeruginosa

Phenylethyl alcohol (PEA) agar plate inoculated with Pseudomonas aeruginosa, a gram-negative bacterium, showing inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

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Phenylethyl Alcohol Agar Inoculated with Proteus mirabilis

Phenylethyl alcohol (PEA) agar plate inoculated with Proteus mirabilis, a gram-negative coliform bacterium, showing inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

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Phenylethyl Alcohol Agar Inoculated with Morexella catarrhalis

Phenylethyl alcohol (PEA) agar plate inoculated with Morexella catarrhalis, a gram-negative bacterium, showing no growth. (Archana Lal, Independence Community College, Independence, KS)

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Phenylethyl Alcohol Agar Inoculated with a Mixture of Gram-Positive and Gram-Negative Bacteria

A phenylethyl alcohol (PEA) agar plate inoculated with a mixture of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.  Only growth of the gram-positive bacteria is seen on the PEA agar.  When inoculated on tryptic soy agar plates, both bacterial types show growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

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Tryptic Soy Agar Inoculated with a Mixture of Gram-Positive and Gram-Negative Bacteria

Tryptic soy agar (TSA) plate inoculated with a mixture of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.  Growth of both bacterial types is seen on the TSA plate.  When this mixture is inoculated on a phenylethyl alcohol (PEA) agar plate, only the gram-positive bacteria shows growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

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Phenylethyl Alcohol Agar Plate

Phenylethyl alcohol (PEA) agar plate inoculated with (a) gram-positive Bacillus subtilis, (b) gram-positive Micrococcus luteus, (c) gram-positive Staphylococcus aureus, and (d) gram-negative Escherichia coli.  The gram-positive bacteria all show good growth while the gram-negative bacterium shows no growth. (Archana Lal, Independence Community College, Independence, KS)

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Uninoculated Phenylethyl Alcohol Agar Plate

An uninoculated phenylethyl alcohol (PEA) agar plate. (Archana Lal, Independence Community College, Independence, KS)

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Infected Human Urine Sample Plated on Sheep Blood Agar and Phenylethyl Alcohol Blood Agar

Infected human urine sample plated on sheep blood agar and phenylethyl alcohol (PEA) blood agar illustrating the power of PEA to separate a gram-positive organism (Staphylococcus saprophyticus) from a gram-negative organism (Escherichia coli). (Rebecca Buxton, University of Utah, Salt Lake City, UT)

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Infected Human Urine Sample Plated on Sheep Blood Agar and Phenylethyl Alcohol Blood Agar (Labeled view)

Infected human urine sample plated on sheep blood agar and phenylethyl alcohol (PEA) blood agar illustrating the power of PEA to separate a gram-positive organism (Staphylococcus saprophyticus) from a gram-negative organism (Escherichia coli). (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

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Staphylococcus saprophyticus from Infected Human Urine Growing on Phenylethyl Alcohol Agar

Staphylococcus saprophyticus from infected human urine growing on phenylethyl alcohol (PEA) agar.  The Escherichia coli known to be present in this sample failed to grow on PEA. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

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Staphylococcus saprophyticus from Infected Human Urine Growing on Phenylethyl Alcohol Agar (Labeled view)

Staphylococcus saprophyticus from infected human urine growing on phenylethyl alcohol (PEA) agar.  The Escherichia coli known to be present in this sample failed to grow on PEA. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

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Sheep Blood Agar Inoculated with Infected Human Urine

Sheep blood agar inoculated with infected human urine showing the growth of two bacterial species, Escherichia coli and Staphylococcus saprophyticus. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

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Sheep Blood Agar Inoculated with Infected Human Urine (Labeled view)

Sheep blood agar inoculated with infected human urine showing growth of two bacterial species, Escherichia coli and Staphylococcus saprophyticus. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

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Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis Streaked on Phenylethyl Alcohol Agar

Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis streaked on phenylethyl alcohol (PEA) agar. PEA agar is selective for gram-positive organisms, particularly gram-positive cocci such as (A) Micrococcus luteus and (B) Staphylococcus epidermidis, which exhibit good growth on the medium. The growth of (C) Klebsiella pneumoniae, a gram-negative organism, is inhibited by the presence of phenylethyl alcohol because phenylethyl alcohol is bacteriostatic for gram-negative bacteria. (Kim R. Finer, Kent State University at Stark, N. Canton, OH)

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Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis Streaked on Phenylethyl Alcohol Agar (Labeled view)

Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis streaked on phenylethyl alcohol (PEA) agar. PEA agar is selective for gram-positive organisms, particularly gram-positive cocci such as (A) Micrococcus luteus and (B) Staphylococcus epidermidis, which exhibit good growth on the medium. The growth of (C) Klebsiella pneumoniae, a gram-negative organism, is inhibited by the presence of phenylethyl alcohol because phenylethyl alcohol is bacteriostatic for gram-negative bacteria. (Labeled view) (Kim R. Finer, Kent State University at Stark, N. Canton, OH)

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Phenylethyl Agar

Bacterial growth on nutrient agar versus phenylethyl alcohol (PEA) agar.  Escherichia coli (left side of plate) and Staphylococcus epidermidis (right side of plate) were plated on nutrient agar (left plate) and PEA agar (right plate). Both plates were incubated at 37oC for 24 hours. On nutrient agar, both cultures grew well.  On PEA agar, however, growth of the gram-negative bacterium E. coli was inhibited, whereas the gram-positive bacterium S. epidermidis flourished. (Clarissa L. Kaup and J. L. Henriksen, Bellevue University, Bellevue, NE)

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Phenylethyl Alcohol Agar

Bacterial growth on phenylethyl alcohol (PEA) agar.  Escherichia coli (left side of plate) and Staphylococcus epidermidis (right side of plate) were plated on PEA agar and incubated at 37°C for 24 hours.  Growth of the gram-negative bacterium E. coli was inhibited, whereas the gram-positive organism S. epidermidis grew without inhibition. (Clarissa L. Kaup and J. L. Henriksen, Bellevue University, Bellevue, NE)

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Phenylethyl Alcohol Agar

Bacterial growth on phenylethyl alcohol (PEA) agar.  Escherichia coli (left side of plate) and Staphylococcus epidermidis (right side of plate) were plated on PEA agar and incubated at 37°C for 24 hours.  Growth of the gram-negative bacterium E. coli was inhibited, whereas the gram-positive organism S. epidermidis grew without inhibition. (Clarissa L. Kaup and J. L. Henriksen, Bellevue University, Bellevue, NE)


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